TABLE I.
Specific binding of [125I]E-IA-DM-PE-PIPZE, in the presence and absence of (−)-cocaine (100 μmol/kg, i.p.), compared to [3H](+)NANM specific binding to σ1 sites in CD-1® mouse brain by ex vivo autoradiography at 60 min
| Brain Region | a Specific Binding [125I]E-IA-DM-PE-PIPZE (fmol/mg tissue) | b Specific Binding [3H](+)-NANM (fmol/mg tissue) | |
|---|---|---|---|
| Control | (−)-Cocaine | ||
| Cortex | |||
| Cingulate | 2.69 ± 0.20 | 1.36 ± 0.14 | 7.3 ± 0.9 |
| Frontal | 2.99 ± 0.19 | 1.58 ± 0.14 | 7.3 ± 0.9 |
| Parietal | 3.12 ± 0.14 | 1.41 ± 0.13 | 8.6 ± 0.7 |
| Temporal | 2.93 ± 0.16 | 1.43 ± 0.14 | 7.5 ± 1.7 |
| Entorhinal | 2.52 ± 0.09 | 1.10 ± 0.11 | 5.3 ± 1.0 |
| Caudate-putamen | 1.96 ± 0.07 | 0.87 ± 0.09 | 5.0 ± 0.5 |
| Hippocampus | |||
| CA1 pyramidal | 2.25 ± 0.12 | 1.04 ± 0.09 | 3.8 ± 1.1 |
| CA2 pyramidal | 2.87 ± 0.13 | 1.41 ± 0.11 | 5.1 ± 1.4 |
| CA3 pyramidal | 3.34 ± 0.10 | 1.50 ± 0.12 | 9.3 ± 1.5 |
| Dentate Gyrus | 3.27 ± 0.08 | 1.39 ± 0.09 | 8.2 ± 2.0 |
| Nonpyramidal | 1.84 ± 0.08 | 0.63 ± 0.09 | 2.2 ± 0.8 |
| Diencephalon | |||
| Thalamus | 2.57 ± 0.13 | 1.52 ± 0.18 | 8.0 ± 0.6 |
| Thalamic nuclei | 2.94 ± 0.10 | 1.60 ± 0.25 | |
| Mesencephalon | |||
| Periaqueductal Gray | 4.07 ± 0.13 | 1.78 ± 0.16 | 11.4 ± 0.8 |
| Superior Colliculi | 3.50 ± 0.14 | 1.59 ± 0.11 | |
| Inferior Colliculi | 3.50 ± 0.20 | 1.69 ± 0.16 | |
| Metencephalon | |||
| Cerebellum (whole) | 3.18 ± 0.08 | 1.32 ± 0.14 | 10.7 ± 0.7 |
| Molecular layer | 2.38 ± 0.08 | 1.08 ± 0.08 | |
| Granular layer | 4.13 ± 0.20 | 1.90 ± 0.15 | |
| White Matter | |||
| Corpus callosum | 1.99 ± 0.06 | 1.29 ± 0.17 | 2.2 ± 0.5 |
a
Values are means ± SEM, n = 4 – 6 from horizontal sections.
b
[3H](+)-NANM data from Bouchard et al., 1996.