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. Author manuscript; available in PMC: 2017 Jan 21.
Published in final edited form as: Mol Cell. 2015 Dec 24;61(2):199–209. doi: 10.1016/j.molcel.2015.12.002

Figure 2. Inducible expression of DN-POLG in HEK293 cells diminishes specific histone H3 acetylation marks.

Figure 2

(A-B) Western blot analysis and quantification of histone 3 acetylation and methylation marks in DN-POLG cells untreated or treated with doxycycline (10 ng/ml) for 3, 6 and 9 days. Mean ± SEM (n=3).

(C) Acetylation state of H3K9 and H3K27 after treating DN-POLG cells at days 0 and 8 of doxycycline (10 ng/ml) treatment with 5 mM sodium acetate (Na-Ace) or 5 mM sodium butyrate (Na-But) for 24h.

(D-E) Quantification of the western blots assessing the acetylation of H3K9 (D) and H2K27

(E) of DN-POLG cells at days 0 and 8 of doxycycline (10 ng/ml) treatment in the presence or absence of 5 mM sodium acetate or sodium butyrate for 24h. Mean ± SEM (n=3).

(F) Acetylation state of H3K9 after treating DN-POLG cells with 5 mM sodium acetate (Na-Ace), 50 μM BMS 303141 (ACL inhibitor) and the combination of both treatments for 48h.

* indicates significance p < 0.05. ** indicates significance p <0.01 throughout Figure 2. See also Figure S2.