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. Author manuscript; available in PMC: 2017 Jan 21.
Published in final edited form as: Mol Cell. 2015 Dec 24;61(2):199–209. doi: 10.1016/j.molcel.2015.12.002

Figure 4. NDI1 and AOX expression in inducible mtDNA depleted cells restores the levels of TCA cycle metabolites.

Figure 4

(A) The heat map displays the fold of change in metabolites involved in important metabolic pathways in DN-POLG-GFP/BFP and DN-POLG-AOX/NDI1 cells treated with doxycycline (10 ng/ml) for 3, 6 and 9 days relative to untreated cells. A yellow-blue color scale depicts the abundance of the metabolites (Yellow: high, Blue: low). Data represent the average of 4 independent experiments.

(B-G) Intracellular citrate (B), fumarate (C), malate (D), α-Ketoglutarate (E), aspartate (F) levels and NAD+/NADH ratio (G) in DN-POLG-GFP/BFP and DN-POLG-AOX/NDI1 cells untreated or treated with doxycycline (10 ng/ml) for 3, 6 and 9 days. Mean ± SEM (n=4).

* indicates significance p < .05. ** indicates significance p <0.01 throughout figure 4. See also Figure S4.