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. Author manuscript; available in PMC: 2017 Jan 21.
Published in final edited form as: Mol Cell. 2015 Dec 24;61(2):199–209. doi: 10.1016/j.molcel.2015.12.002

Figure 5. Restoration of the oxidative TCA cycle activity in inducible mtDNA depleted cells is sufficient to rescue specific histone 3 acetylation marks but not cell proliferation.

Figure 5

(A-B) DN-POLG-GFP/BFP and DN-POLG-AOX/NDI1 cells were grown in the presence or absence of doxycycline (10 ng/ml) for 12 days and cell number was assessed. Cells were supplemented with pyruvate and uridine in the presence (B) or absence (A) of aspartate (20 mM). Mean ± SEM (n=3).

(C) Western blot analysis of p-AMPK, AMPK and Tubulin in DN-POLG-AOX/NDI1 untreated or treated with doxycycline (10 ng/ml) for 3, 6 and 9 days.

(D) Quantification of the western blots assessing the phosphorylation state of AMPK in DN-POLG-AOX/NDI1. Data is represented relative to total levels of AMPK and the mean value in untreated cells. Mean ± SEM (n=3).

(E-G) Western blot analysis and quantification of the acetylation of H3K9, H3K18 and H3K27 and the total expression of H3 in DN-POLG-GFP/BFP and DN-POLG-AOX/NDI1 cells untreated or treated with doxycycline (10 ng/ml) for 9 days. Data was normalized to the mean value in untreated cells. Mean ± SEM (n=3).

* indicates significance p < .05. ** indicates significance p <0.01 throughout figure 5. See also Figure S5.