. 2016 Jan 23;6(1):36. doi: 10.1007/s13205-015-0353-y

Table 4.

Effect of soaking time, repeated extraction and various extractants on pectinase recovery

Extraction	Pectinase activity
Extraction	Ugds⁻¹	Umg⁻¹	Ug⁻¹
Soaking time (h)
0.5	2500 ± 710^f	963 ± 270^h	500 ± 140^g
1	3500 ± 710^e	1251 ± 190^g	700 ± 140^f
3	4500 ± 710^d	1954 ± 300^f	900 ± 140^e
5	5500 ± 710^c	2574 ± 220^e	1100 ± 140^d
7	7500 ± 710^b	3736 ± 220^c	1500 ± 140^b
17	6500 ± 710^bc	3592 ± 690^c	1300 ± 140^c
24	5500 ± 710^c	2541 ± 260^e	1100 ± 140^cd
48	12,500 ± 710^a	5297 ± 420^a	2500 ± 140^a
72	12,000 ± 0^a	4727 ± 130^b	2400 ± 140^a
96	11,500 ± 710^a	4652 ± 230^ab	2500 ± 140^a
Repeated extraction
1	6500 ± 710^bc	870.8 ± 270ⁱ	700 ± 140^f
2	7500 ± 710^b	3280 ± 410^d	1500 ± 140^b
3	5500 ± 710^c	3726 ± 210^c	1100 ± 140^d
Different extractants
PB (0.1 M)	5500 ± 710^c	1992 ± 310^f	1100 ± 140^d
PB +Trit × 100 (0.1 %, w/v)	4500 ± 710^d	2066 ± 280^ef	900 ± 140^e
PB +Trit × 100 (0.5 %, w/v)	3500 ± 710^e	1187 ± 210^gh	700 ± 140^f
PB +Trit × 100 (1 %, w/v)	3000 ± 710^ef	865.4 ± 310ⁱ	600 ± 140^fg
PB + Urea (1 M)	2500 ± 710^f	599.4 ± 340^j	500 ± 140^g
PB + Amm. Sulfate (1 M)	3500 ± 710^de	1260 ± 220^g	700 ± 140^f
NaCl (0.5 %)	2500 ± 710^f	1098 ± 180^h	500 ± 140^g
DW	3500 ± 710^d	993 ± 230^h	700 ± 140^f
Czapek Dox	3000 ± 710^e	886 ± 120ⁱ	600 ± 140^fg

The solid state fermentation setups were performed for a period of 96 h at 30 °C. ND not determined. The data represent mean ± SD of replicates (n = 3). Mean values within a column with different upper-script letters are significantly different at p ≤ 0.05