Fig. 1.

Schematic drawing of the experimental design. a Observation of hyphal contacts between two clusters of spores from different isolates b Identification of spores produced in anastomosis regions and inoculation of a single spore on a new petri dish with Ri t-DNA transformed chicory roots i.e. monosporal cultures. After germination and colonisation of the petri dish, spores are extracted and their DNA is used for genotyping of the monosporal culture