Figure 4. Tumor-infiltrating TCR1045 T cells have a phenotypic signature consistent with antigen recognition and are dysfunctional.
(A) Donor TCR1045 cell frequency 28 days post T cell transfer. Plots are gated on CD45+CD8+ T cells.
(B) Donor TCR1045 cell frequency in spleens (Spl) and tumors 8 (D8) or 28 (D28) days post T cell transfer.
(C) Number of donor TCR1045 cells 8 or 28 days post T cell transfer..
(D) Tetramer staining of splenic and intratumoral donor TCR1045 cells 28 days post transfer.
(E) Phenotype of donor (CD8+Thy1.1+) TCR1045 cells compared to concurrently isolated splenic host (CD8+Thy1.1−) T cells 28 days post transfer.
(F) Frequency or MFI of donor T cells positive for indicated antigens in spleen and PDA. Mean ± SEM, n=3 each.
(G) Inhibitory receptor expression by donor TCR1045 cells and endogenous T cells.
(H) Frequency of donor TCR1045 cells expressing indicated molecules 28 days post T cell transfer.
(I) Ex vivo cytokine production by T cells in presence (+) or absence (−) of antigen 8 days post T cell transfer.
(J) Donor T cell frequencies producing both IFNγ and TNFα after 5 hr restimulation with antigen.
Data are shown as mean ± SEM. See also Figure S4.