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. Author manuscript; available in PMC: 2016 Nov 9.
Published in final edited form as: Cancer Cell. 2015 Oct 29;28(5):638–652. doi: 10.1016/j.ccell.2015.09.022

Figure 4. Tumor-infiltrating TCR1045 T cells have a phenotypic signature consistent with antigen recognition and are dysfunctional.

Figure 4

(A) Donor TCR1045 cell frequency 28 days post T cell transfer. Plots are gated on CD45+CD8+ T cells.

(B) Donor TCR1045 cell frequency in spleens (Spl) and tumors 8 (D8) or 28 (D28) days post T cell transfer.

(C) Number of donor TCR1045 cells 8 or 28 days post T cell transfer..

(D) Tetramer staining of splenic and intratumoral donor TCR1045 cells 28 days post transfer.

(E) Phenotype of donor (CD8+Thy1.1+) TCR1045 cells compared to concurrently isolated splenic host (CD8+Thy1.1) T cells 28 days post transfer.

(F) Frequency or MFI of donor T cells positive for indicated antigens in spleen and PDA. Mean ± SEM, n=3 each.

(G) Inhibitory receptor expression by donor TCR1045 cells and endogenous T cells.

(H) Frequency of donor TCR1045 cells expressing indicated molecules 28 days post T cell transfer.

(I) Ex vivo cytokine production by T cells in presence (+) or absence (−) of antigen 8 days post T cell transfer.

(J) Donor T cell frequencies producing both IFNγ and TNFα after 5 hr restimulation with antigen.

Data are shown as mean ± SEM. See also Figure S4.

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