Fig. 2. Labeling of Gpx3-SOH with various nucleophiles under oxidizing conditions. Gpx3-SH (10 μM) was incubated with various nucleophiles at 100 μM or 1 mM concentration under oxidizing (1.5 eq. H2O2) conditions for 1 h and analyzed by LTQ-MS. (A) Reduced and oxidized Gpx3; (B) dimedone (1); (C) 1-benzylpiperidine-2,4-dione 26f; (D) 1-benzyl-1H-benzo[c][1,2]thiazin-4(3H)-one 2,2-dioxide 31h; (E) 1,3-indandione 34b; (F) N-methylbarbituric acid 27b; (G) isothiochroman-4-one 2,2-dioxide 31b; (H) 1-benzylpyrrolidine-2,4-dione 32c; (I) 2-benzylisothiazolidin-4-one 1,1-dioxide 33f; (J) 2-benzyl-2H-1,2-thiazin-5(6H)-one 1,1-dioxide 31f; (K) 2-isopropyl-2H-1,2-thiazin-5(6H)-one 1,1-dioxide 31e; (L) ((1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-yl)methanol 5.
