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. Author manuscript; available in PMC: 2017 Feb 10.
Published in final edited form as: J Control Release. 2015 Dec 22;223:126–136. doi: 10.1016/j.jconrel.2015.12.031

Figure 5.

Figure 5

DNA content (a), CD31 protein expression (b), representative western blot bands (c), and mRNA expression of vasculogenic markers vWF (d), CD31 (e), and VE-cadherin (f) for hMSCs and ECFCs encapsulated in the patterned constructs. Groups included patterned constructs without VEGF/BMP2 (green), with VEGF/BMP2 (red), and with NG-VEGF/NG-BMP2 (blue); unpatterned vasculogenic constructs with NG-VEGF (dashed light blue) and VEGF (dashed pink). (g) CD31 stained (green) images of the patterned constructs along the length of a microchannel with None, directly added, and NG-grafted BMP2/VEGF after 4 and 10 days of incubation. “An asterisk” represents a statistically significant difference in the ordinate between the test and None groups for the same time point; “Two asterisks” represents a significant difference between the test group and the patterned construct with VEGF/BMP2. Error bars correspond to means ± 1 SD for n=3.