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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Immunol. 2015 Dec 30;196(3):1366–1375. doi: 10.4049/jimmunol.1401838

Figure 2. miR-34a negatively regulates efferocytosis by human AMø.

Figure 2

AMø were harvested by bronchoalveolar lavage of healthy human never-smoker volunteers and transfected in vitro using control (scrambled) or miR-34a-specific antagomirs using RNAiMAX lipofectamine. Following transfection, AMø were either (A) processed to isolate total RNA, which was assayed by quantitative real-time PCR; or (B) exposed to AC for 90 min and then assayed for efferocytosis in chamber slides, which were H&E-stained and counted by microscopy. Data are mean ± SEM of individual subjects in independent experiments, A, n=3; B, n=4; *, p<0.05 by one-way ANOVA with Bonferroni post-hoc testing.