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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Immunol. 2015 Dec 30;196(3):1366–1375. doi: 10.4049/jimmunol.1401838

Figure 6. Efferocytosis down-regulates miR-34a, creating a positive-feedback loop for secondary engulfment.

Figure 6

A. Resident AMø from wt mice were exposed to AC f for 2 h, then AC were removed by vigorous washing and AMø were incubated in LCM for a further 22 h before a second aliquot of AC were added and efferocytosis was assayed after 90 min using a microscopy assay of H&E stained slides. B. Resident AMø from wt mice were exposed to AC or LCM for 24 h, washed to remove unbound AC and then total RNA was harvested; expression of miR-34a, relative to sno-142, was assessed using quantitative real-time RT-PCR. C. Resident PMø from wt mice were exposed to AC twice, exactly as in panel A, except that in the second exposure, AC were TAMRA-labeled and efferocytosis was assayed after 60 min using flow cytometry. C. Data in all graphs are mean ± SEM from at least four replicates from two or more independent experiments. *, p<0.05; **, p<0.01 by Student t test.