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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: Dev Biol. 2015 Nov 30;409(2):406–419. doi: 10.1016/j.ydbio.2015.11.022

Figure 8. ND mutant disrupts the tubulin heterodimer.

Figure 8

(A) Tubulin biogenesis pathway in yeast. Adapted from (Lewis et al., 1997; Nithianantham et al., 2015). (B) Overexpression analysis. Strains with indicated genotypes containing plasmids for overexpressing TBCD/CIN1 or TBCA/RBL2 were grown to saturation at 30°C in non-inducing media and a 10-fold dilution series of each was spotted to inducing media. Plates were grown at 30°C for 3 days. Strains: wild type, yJM0597; tub1-ND tub3-ND, yJM0714; tub3Δ, yJM0120.