Table 1.
Strain | Mean length (μm) |
Polym rate (μm/min) |
Polym duration (sec) |
Depolym Rate (μm/min) |
Depolym duration (sec) |
Rescue frequency (events/min) |
Catastrophe frequency (events/min) |
---|---|---|---|---|---|---|---|
Wild type | 1.1 ± 0.01 | 1.8 ± 0.1 | 43 ± 2 | 3.0 ± 0.1 | 28 ± 1 | 1.2 ± 0.08 | 0.9 ± 0.05 |
tub1-ND | 1.1 ± 0.02 | 2.1 ± 0.1 | 36 ± 2 | 3.6 ± 0.2 | 24 ± 1 | 1.2 ± 0.11 | 1.1 ± 0.08 |
tub1-ND tub3-ND | 1.1 ± 0.01 | 1.8 ± 0.1 | 44 ± 3 | 3.4 ± 0.1 | 26 ± 1 | 1.0 ± 0.1 | 0.8 ± 0.07 |
Polym, polymerization; depolym, depolymerization; μm, micron; min, minute; sec, second. Values are mean ± SEM of measurements from at least 32 microtubules in cells imaged for at least 240 seconds at 4 second intervals. Values in bold italics are significantly different from wild-type controls (p<0.05), based on a students t-test.