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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: Dev Biol. 2015 Dec 3;409(2):382–391. doi: 10.1016/j.ydbio.2015.11.024

Figure 1. Nephron progenitor PRR is required for nephrogenesis.

Figure 1

Efficiency of Six2-Cre-mediated PRR deletion. (A) PRR mRNA levels are reduced in Six2+ cells FACS-isolated from E15.5 Six2PRR−/− kidneys compared with controls. (B,C) PRR immunostaining (red) is reduced in the mesenchyme of Six2PRR−/− E15.5 kidneys. Ureteric buds (UB) are visualized with anti-pancytokeratin antibody (green staining). (D–I) 3D reconstruction of E12.5 kidneys demonstrates that mutant kidneys have no nephrons past the RV stage (light blue), while control kidneys have RVs, comma (red) and SBs (dark purple). Both Six2PRR−/− and control kidneys show robust UB branching (pink). (D–I) Scale bars= 100 µm.