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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Phytochemistry. 2015 Nov 25;122:11–21. doi: 10.1016/j.phytochem.2015.11.002

Table 1.

Kinetic parameters of the K. brevis EH with various substrates and inhibitors

Entry Substrate Km (μM) kcat (sec−1) kcat (sec−1)/Km (M) IC50 (mM)
1 Probe 5a (R = Ph) 13.6 7.81E-4 57.4 -
2 Probe 5b (R = Et) 161 4.46E-3 27.7 -
3 (R) styrene oxide 6240 9.01E-1 144 -
4 (S) styrene oxide 6430 8.32E-1 129 -
5 (R/S) styrene oxide 6570 8.58E-1 131 -
6 α-methylstyrene oxide 5310 3.31E-1 62.3 -
7 (R) 1-butane oxide 25,800 6.15 238 -
8 (R/S) 1-butane oxide 22,200 5.36 241 -
9 1,2,7,8-diepoxyoctane 4,800 7.12 1483 -
10 cis-β-methylstyrene oxide - - - 3.05
11 trans-β-methylstyrene oxide- - - - 14.28
12 β-dimethylstyrene oxide - - - 6.99
13 cis-2,3-epoxybutane - - - -
14 2,3-dimethyl-2,3-epoxybutane - - - −3