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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Immunol. 2016 Jan 6;196(3):1044–1059. doi: 10.4049/jimmunol.1501962

Figure 3. Administration of α-GalCer induces an expansion of activated CD1d-restricted iNKT cells in decidual tissues, which is blunted by rosiglitazone.

Figure 3

(A) Gating strategy used to identify CD1d-restricted iNKT cells (CD1d tetramer+DX5+NK1.1+TCRβ+ cells) in decidual tissues. (B) Number of CD1d-restricted iNKT cells in decidual tissues from mice injected with DMSO, α-GalCer, rosiglitazone, or α-GalCer + rosiglitazone. Data are from individual dams, n=6–8 each. (C) Immunophenotyping of activation markers CD69, CD44, and IL4 in CD1d-restricted iNKT cells in decidual tissues from mice injected with DMSO, α-GalCer, rosiglitazone (Rosi), or α-GalCer + rosiglitazone. The gray histogram represents the autofluorescence control, and the white histogram represents the fluorescence signal from CD1d-restricted iNKT cells. (D&E) Number of CD69+CD44+ or IL4+CD1d-restricted iNKT cells in decidual tissues from mice injected with DMSO, α-GalCer, rosiglitazone, or α-GalCer + rosiglitazone. Data are from individual dams, n=6–8 each.