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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Immunol. 2015 Dec 30;196(3):1412–1418. doi: 10.4049/jimmunol.1501886

FIGURE 2. TWIST1 expression positively correlates with C3 expression.

FIGURE 2

(A) Downregulation of TWIST1 by transfecting human ovarian cancer cells (SKOV3ip1 and OVCAR 5) with TWIST1 siRNAs was detected by measuring TWIST1 mRNA. (B) Subsequent to TWIST1 knockdown in C3-high SKOV3ip1 and OVCAR 5 cells, C3 expression was measured by quantitative RT-PCR. TWIST1 knockdown resulted in 99% reduction in C3 expression (n=3, p=0.001). (C) TWIST1 overexpression in C3-low HeyA8 and A2780 ovarian cancer cells by stable transfection of plasmid encoding TWIST1 cDNA (pTWIST1) resulted in 2 folds and 75 folds increase in TWIST1 expression, respectively (n=3, p=0.0001). (D) C3 mRNA levels in HeyA8 and A2780 cells stably transfected with the TWIST1 cDNA was measured by quantitative RT-PCR. TWIST1 overexpression in HeyA8 and A2780 cells resulted in 1.7 and 8.8 folds increase in C3 expression, respectively (n=3, p<0.005)

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