Skip to main content
. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Immunol. 2016 Jan 4;196(3):1355–1365. doi: 10.4049/jimmunol.1500793

Figure 12. Annexin A2 increases clearance of S. pneumonia in acute otitis media.

Figure 12

A) S. pneumonia (Spn 6A) was incubated with 50% normal human serum (NHS) with or without the addition of annexin A2 (2 μg/ml). C3b deposition on the bacteria was measured by flow cytometry and was significantly greater when annexin a2 was added to the reaction. B) Spn 6A was incubated with 50% normal mouse serum (NMS) or serum from factor B deficient mice (fB−/−). C3b deposition on the bacteria was measured by flow cytometry and was significantly greater when annexin a2 was added to NMS. The addition of annexin A2 did not increase C3b deposition on the bacteria when incubated with serum from fB−/− mice. C) Spn 6A was incubated with fluorescently labeled annexin A2 which bound to a subset of the cells. D) Spn 6A was incubated with fluorescently labeled factor H and recombinant annexin A2. The addition of annexin A2 reduced binding of factor H to the bacteria. Each experiment was performed three times. **P<0.01, ***P< 0.001.