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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Immunol. 2016 Jan 4;196(3):1355–1365. doi: 10.4049/jimmunol.1500793

Figure 5. Annexin A2 binds factor H on solid surfaces.

Figure 5

A) Annexin A2 was immobilized on an ELISA plate and incubated with factor H. Factor H bound to the immobilized annexin A2. The experiment was repeated three times, and representative results are shown. B) Surface plasmon resonance was performed. Factor H was immobilized on a CM5 chip and the binding of annexin A2 was evaluated at several different concentrations (3 μM, 1 μM, 333 nM, 111 nM, and 37 nM). Annexin A2 bound to the factor H with a dissociation constant of approximately 7 μM.