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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Immunol. 2015 Dec 23;196(3):1305–1316. doi: 10.4049/jimmunol.1501171

Figure 2. CD43, PSGL-1, but not CD44, function as Th17 cell E-selectin ligands under shear flow conditions in vitro.

Figure 2

Th17 (A, C) and Th1 (B, D) cells (1 × 106 cells/ml) generated in vitro from the indicated deficient mice were perfused at 1 dyne/cm2 over glass coverslips coated with recombinant E-selectin (A, B) or P-selectin (C, D). n=8 independent Th17 and Th1 preparations for WT mice; n= 5 independent cell preparations for PSGL1−/−CD43−/− DKO and PSGL1−/−CD44−/− DKO; n= 4 independent cell preparations for CD43−/−. All groups were studied side by side in at least n=3 independent experiments. Each group was studied side by side with WT cells in n= 4 or n= 5 independent experiments. E. Th17 and Th1 cells were sorted based on the level of CD43 expression in three groups: CD43high for Th17 cells, and CD43 intermediate (CD43M) or CD43high (CD43H) for Th1 cells. F. Th17 cells, Th1 CD43M and Th1 CD43H were perfused at a shear stress of 1 dyne/ cm2 over E-selectin coated coverslips. n= 3 independent experiments with 3 different T cell subset preparations. Data show the mean ± SD values. *p<0.05, **p<0.01, ***p<0.001 as compared with WT or as indicated.