Skip to main content
. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Immunol. 2015 Dec 23;196(3):1081–1090. doi: 10.4049/jimmunol.1500719

FIGURE 2.

FIGURE 2

LZTFL1 localizes on the cell membrane in an F-actin-dependent manner. (A) Human CD4+ T cells isolated from healthy donors were transiently transfected with LZTFL1-Flag expression DNA. Cells were fixed and stained with anti-Flag Ab for LZTFL1-Flag (green). Cholera toxin B and DAPI were used to stain the cytoplasmic membrane marker GM1 ganglioside (red) and the nucleus (blue), respectively, and the staining was analyzed by confocal microscopy. (B) Fluorescent images of Jurkat T cells stained with anti-LZTFL1 Ab (green) and DAPI (blue). (C) Fluorescent images of Halo-LZTFL1-expressing Jurkat T cells treated with or without latrunculin B (1 μM) for 30 min. Cells were stained with phalloidin for F-actin (green), anti-halo Ab for LZTFL1 (red), and DAPI for the nucleus (blue).