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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Immunol. 2015 Dec 23;196(3):1081–1090. doi: 10.4049/jimmunol.1500719

FIGURE 6.

FIGURE 6

LZTFL1 enhances T cell activation by specifically upregulating NFAT activity. (A) Jurkat cells were cotransfected with DNAs encoding LZTFL1-Flag and NFAT-Luc or LZTFL1-Flag and NFkB-Luc for 2 d. Renilla luciferase, under the control of the thymidine kinase promoter, was used as a transfection control. Cells were activated with anti-CD3 and anti-CD28 Abs-bound beads for 10 h. The firefly luciferase to Renilla luciferase ratio was calculated, and levels relative to those of the pCMV6 empty vector–transfected and unactivated cells were plotted (mean ± SD). n = 5. *, p value < 0.05 (comparison to cells transfected with pCMV6 empty vector and unactivated); #, p < 0.05 (comparison to cells transfected with pCMV6 empty vector and activated). (B) Jurkat cells cotransfected with reporter NFAT-Luc and an increasing amount of LZTFL1 expression DNA. Cells were activated, and the relative firefly luciferase to Renilla luciferase ratio was plotted as in (A) (mean ± SD). n = 5. *, p value < 0.05 (comparison to cells transfected with pCMV6 empty vector and unactivated); #, p value < 0.05 (comparison to cells transfected with pCMV6 empty vector and activated). The expression of endogenous and transfected LZTFL1 was analyzed by Western blotting.