The shapes of leading edges of keratocytes associated with three fish species. (A–C) Three typical cells from a cichlid (A), goldfish (B), and black tetra (C). (A)–(C) are typical of 103, 101, and 98 cells, respectively. (D–L) Evaluation of the shape of the leading edge. (D) Definition of radius of curvature and center angle of the leading edge. See text for details. (E–G) Frequency distribution of radius of curvature of keratocytes from a cichlid (E), goldfish (F), and black tetra (G). (H–J) Frequency distribution of center angle of keratocytes from a cichlid (H), goldfish (I), and black tetra (J). (K and L) Average radii of curvature and center angles calculated from (E)–(G) and (H)–(J), respectively. The p-values were calculated using the Steel-Dwass test.