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. 2016 Jan 19;110(2):481–492. doi: 10.1016/j.bpj.2015.12.014

Figure 2.

Figure 2

Rates of LEE, ARF, and AP. (A–J) Fluorescence speckle observation of ARF in keratocytes loaded with Alexa Fluor 546 phalloidin. (A–C) Single fluorescence images selected from consecutive images. (A)–(C), are typical of 22, 19, and 16 cells, respectively. (D) Definition of the middle, side 1, side 2, and side 3 directions. See text for details. (E–G) Kymographs constructed from image strips, with width 8 pixels (= 1 μm) (white rectangles labeled as m in A–C) from consecutive images taken at 2-s intervals. (H–J) Kymographs from image strips (s1 in A–C). Movements of the cell edge and ARF are indicated with white and yellow arrows, respectively. (K and L) Rates of the LEE (top of K), ARF (bottom of K), and AP (L) at the middle, side 1, side 2, and side 3 (red, green, blue, and cyan columns, respectively) in the wide, intermediate, and round cells. The kymographs in (L) were calculated as the sum of those in (K). Straight lines in (K) are rate gradients of the LEE and ARF. The p-values compared with middle were calculated using the Tukey-Kramer test. To see this figure in color, go online.