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. 2016 Jan 19;110(2):481–492. doi: 10.1016/j.bpj.2015.12.014

Figure 3.

Figure 3

Distribution of myosin IIA, vinculin, and stress fibers. (A–F) Immunofluorescence staining of myosin IIA (A–C; white) and vinculin (D–F; green) in a wide (A and D), intermediate (B and E), and round cell (C and F). (A)–(F) are typical of 37, 32, 32, 37, 39, and 37 cells, respectively. (G–I) F-actin stained with Alexa Fluor 488 phalloidin (magenta). Green arrows indicate stress fibers. The cells in (G)–(I) are the same as those in (D)–(F), respectively. (G)–(I) are typical of 37, 39, 37 cells, respectively. (J–L) Merge images of (D)–(F) and (G)–(H), respectively. Areas of colocalization of vinculin and F-actin appear white. (M) Definition of middle (mw) and side (sw) regions. See text for details. (N) The ratio of the density of myosin IIA in sw to that in mw in the three fish species (A–C). (O) The ratio of the density of vinculin in sw to that in mw in the three fish species (D–F). (P) The ratio of the posterior vinculin area (green ellipses in D–F) to the whole cell area in the three cell groups. The p-values in (N)–(P) were calculated using Welch’s t-test and Steel-Dwass test, respectively. To see this figure in color, go online.