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. 2016 Jan 19;110(2):481–492. doi: 10.1016/j.bpj.2015.12.014

Figure 5.

Figure 5

Decrease of radius of curvature in response to the application of trypsin-EDTA. (A) Sequential images of a crawling wide cell. Trypsin-EDTA was applied to the left side of the cell through a glass microneedle. Images in (A) are typical of 10 experiments. (B) Sequential images of fluorescence of Fluo-4, which was included in the applied solution. The images of (A) and (B) were taken simultaneously. (C) Fluorescence intensity along the line in (B). (D) Cell outlines of every 10 s made from (A). Gray and black arrows indicate the contraction of the cell. (E) Sequential images of a crawling wide cell. Culture medium was applied to the left side of the cell. Images in (E) are typical of 10 experiments. (F) Sequential images of fluorescence of Fluo-4. The images of (E) and (F) were taken simultaneously. (G) Fluorescence intensity along the line in (F). (H) Cell outlines of every 10 s made from (E). (I) Ratio of fluorescence intensity near the tip of the application needle to that at the opposite side. (J) Radii of curvature of the leading edges in dh and ah, measured 70% into the spray period. Definition of dh and ah (inset). See text for details. (K and L) Radii of curvature (K) and center angles (L) of leading edge before (gray) and after (white) the application of trypsin-EDTA or culture medium. The p-values were calculated using Welch’s t-test. To see this figure in color, go online.