Figure 7.

Rise in ARF rate in response to the ablation of stress fibers. (A) Sequential images of F-actin in a crawling cichlid keratocyte. At 0 s, a proportion of stress fibers was ablated by laser microablation (yellow arrow). Images in (A) are typical of 11 experiments. (B) Cell outlines of every 5 s made from (A). (C) Definitions of ablated side and SF side. Purple lines: stress fibers. See text for details. (D and E) Kymographs constructed from image strips, with width 10 pixels (= 1.3 μm) (white rectangles labeled as ab and sf in A) from consecutive images taken at 5-s intervals. The ab and sf are in the direction of the ablated and SF sides, respectively. Movements of the cell edge and ARF are indicated with white and yellow arrows, respectively. (F) ARF rates at the ablated and SF sides from 11 cells. It should be noted that the rate is faster in the lower part of the graph. The data in the (D) and (E) are indicated as blue squares in F. To see this figure in color, go online.