Table 2.
Determination of IC50 values of the selected compounds in PMNL, HWB and in the enzymatic assay.
| Compd. | IC50/μmol/L |
||
|---|---|---|---|
| PMNL | HWB | Enzymatic assaya | |
| 4c | 0.45 | 4.87 | 0.136±0.015 |
| 4e | 0.69 | 1.08 | 0.133±0.014 |
| 4g | 0.75 | 2.11 | 0.165±0.017 |
| 4j | 1.07 | 33.9 | −b |
| 4k | 0.90 | 5.83 | 0.159±0.016 |
| 5e | 0.64 | 0.94 | 0.169±0.016 |
| 5f | 0.81 | 4.65 | 0.273±0.021 |
| 5i | 0.80 | 1.13 | 0.127±0.029 |
| 6b | 0.82 | 10.2 | 0.327±0.022 |
| Zileuton | 1.19 | 4.83 | 0.206 |
HWB, human whole blood; PMNL, rat peritoneal polymorphonuclear leukocytes.
a
Each concentration was tested in triplicate. The IC50 was calculated with the sigmoidal dose-response (variable slope) fitting equation in GraphPad Prism.
b
Not determined.