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. 2015 Dec 11;107(1):18–27. doi: 10.1111/cas.12844

Figure 4.

Figure 4

Long non‐coding RNA urothelial cancer‐associated 1 (lncRNA‐UCA1) promotes bladder cancer cell migration and invasion through the hsa‐miR‐145–fascin homologue 1 (FSCN1) pathway. (a) Quantitative PCR analysis of miRNA expression levels in UMUC2 cells transfected with pcDNA3.1 or pcDNA3.1‐UCA1. (b) Quantitative PCR analysis of lncRNA‐UCA1 and hsa‐miR‐145 RNA levels in UMUC2 cells transfected with pcDNA3.1 and pcDNA3.1‐UCA1 at a final concentration of 0.5, 1, and 2 μg. (c, d) Quantitative PCR analysis of lncRNA‐UCA1 and hsa‐miR‐145 RNA levels in 5637 and T24 cells transfected with shRNA control or lncRNA‐UCA1 shRNA. (e, f) Quantitative PCR and Western blot analyses of lncRNA‐UCA1 and FSCN1 expression levels in UMUC2 cells transfected with pcDNA3.1 or pcDNA3.1‐UCA1 and 5637 cells transfected with shRNA control or lncRNA‐UCA1 shRNA. (g) Correlation analysis of lncRNA‐UCA1 and hsa‐miR‐145 expression levels in human bladder cancer specimens (n = 25). All data are the averages of at least three independent experiments and data are presented as mean ± SD. *P < 0.05.