Table 2.
Gene | Sequence (5′–3′) | Experimental use |
---|---|---|
UCA1 | CTCTCCATTGGGTTCACCATTC | Real‐time PCR |
GCGGCAGGTCTTAAGAGATGAG | ||
E‐cadherin | GCTGCTCTTGCTGTTTCTTCG | Real‐time PCR |
CCGCCTCCTTCTTCATCATAG | ||
Vimentin | AAGTTTGCTGACCTCTCTGAGGCT | Real‐time PCR |
CTTCCATTTCACGCATCTGGCGTT | ||
N‐cadherin | GCCCCTCAAGTGTTACCTCAA | Real‐time PCR |
AGCCGAGTGATGGTCCAATTT | ||
ZEB1 | TTCAAACCCATAGTGGTTGCT | Real‐time PCR |
TGGGAGATACCAAACCAACTG | ||
ZEB2 | CGCTTGACATCACTGAAGGA | Real‐time PCR |
CTTGCCACACTCTGTCCATT | ||
Snail1 | TCCAGAGTTTACCTTCCAGCA | Real‐time PCR |
CTTTCCCACTGTCCTCATCTG | ||
Snail2 | CTACAGCGAACTGGACACACA | Real‐time PCR |
GCCCCAAAGATGAGGAGTATC | ||
Twist1 | GTCCGCAGTCTTACGAGGAG | Real‐time PCR |
GTCTGAATCTTGCTCAGCTTGT | ||
Twist2 | ACAAGCTGAGCAAGATCCAGAC | Real‐time PCR |
GCTGGTCATCTTATTGTCCATC | ||
FSCN1 | GGCAAGTTTGTGACCTCCAAGAA | Real‐time PCR |
AGCCGATGAAGCCATGCTC | ||
ACTB (β‐actin) | TCCCTGGAGAAGAGCTACGA | Real‐time PCR |
AGCACTGTGTTGGCGTACAG | ||
miR‐145 binding site | GCCATATGAAGACACCCTCGATCAGTCC | Site‐directed mutagenesis |
GGACTGATCGAGGGTGTCTTCATATGGC | ||
UCA1 shRNA control | gatccTTCTCCGAACGTGTCACGTttcaagaga | RNA interference |
ACGTGACACGTTCGGAGAAttttttggaaa | ||
UCA1 shRNA | gatccGTTAATCCAGGAGACAAAGAttcaaga | RNA interference |
gaTCTTTGTCTCCTGGATTAACttttttggaaa |