Table 2.
Primers and shRNAs used in this study of bladder cancer cell migration and invasion
| Gene | Sequence (5′–3′) | Experimental use |
|---|---|---|
| UCA1 | CTCTCCATTGGGTTCACCATTC | Real‐time PCR |
| GCGGCAGGTCTTAAGAGATGAG | ||
| E‐cadherin | GCTGCTCTTGCTGTTTCTTCG | Real‐time PCR |
| CCGCCTCCTTCTTCATCATAG | ||
| Vimentin | AAGTTTGCTGACCTCTCTGAGGCT | Real‐time PCR |
| CTTCCATTTCACGCATCTGGCGTT | ||
| N‐cadherin | GCCCCTCAAGTGTTACCTCAA | Real‐time PCR |
| AGCCGAGTGATGGTCCAATTT | ||
| ZEB1 | TTCAAACCCATAGTGGTTGCT | Real‐time PCR |
| TGGGAGATACCAAACCAACTG | ||
| ZEB2 | CGCTTGACATCACTGAAGGA | Real‐time PCR |
| CTTGCCACACTCTGTCCATT | ||
| Snail1 | TCCAGAGTTTACCTTCCAGCA | Real‐time PCR |
| CTTTCCCACTGTCCTCATCTG | ||
| Snail2 | CTACAGCGAACTGGACACACA | Real‐time PCR |
| GCCCCAAAGATGAGGAGTATC | ||
| Twist1 | GTCCGCAGTCTTACGAGGAG | Real‐time PCR |
| GTCTGAATCTTGCTCAGCTTGT | ||
| Twist2 | ACAAGCTGAGCAAGATCCAGAC | Real‐time PCR |
| GCTGGTCATCTTATTGTCCATC | ||
| FSCN1 | GGCAAGTTTGTGACCTCCAAGAA | Real‐time PCR |
| AGCCGATGAAGCCATGCTC | ||
| ACTB (β‐actin) | TCCCTGGAGAAGAGCTACGA | Real‐time PCR |
| AGCACTGTGTTGGCGTACAG | ||
| miR‐145 binding site | GCCATATGAAGACACCCTCGATCAGTCC | Site‐directed mutagenesis |
| GGACTGATCGAGGGTGTCTTCATATGGC | ||
| UCA1 shRNA control | gatccTTCTCCGAACGTGTCACGTttcaagaga | RNA interference |
| ACGTGACACGTTCGGAGAAttttttggaaa | ||
| UCA1 shRNA | gatccGTTAATCCAGGAGACAAAGAttcaaga | RNA interference |
| gaTCTTTGTCTCCTGGATTAACttttttggaaa |