Figure 5.

Proliferation and chemosensitivity assay results in TC616 soft tissue leiomyosarcoma cell lines with FOXM1. The cell lines were transduced with FOXM1 siRNA (si FOXM1) or a non‐targeting control (si control). (a) Western blot analysis showed that the cell lines transduced with FOXM1 had significantly reduced levels of FOXM1 protein at 48 h after transfection. (b) Real‐time quantitative PCR for FOXM1 showed a reduction in FOXM1 transcript at 24 h after transfection. (c) TC616 cells with FOXM1 siRNA compared to non‐targeting control. Significantly decreased proliferation was recognized in the TC616 cells at 96 h after transfection. (d) SiRNA targeting FoxM1 transfected cells had higher sensitivity for doxorubicin (DOX), compared with the control. Data are presented as mean ± SD for three independent experiments. *P < 0.05, t‐test.