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. 2015 Dec 8;109(2):318–330. doi: 10.1093/cvr/cvv268

Figure 3.

Figure 3

TIMP-2 deficiency retards monocyte/macrophage invasion. (A) Representative CD11b IHC-labelled Matrigel-coated invasion inserts and quantification of TIMP-1+/+, TIMP-1−/−, TIMP-2+/+ and TIMP-2−/− monocyte invasion in vitro (n = 4; *P < 0.05 denotes significant difference from all other groups). Scale bar represents 50 µm and is applicable to all panels. (B) Representative CD11b IHC-labelled images and quantification of macrophage number within subcutaneous sponges from TIMP-1+/+, TIMP-1−/−, TIMP-2+/+ and TIMP-2−/− mice, and TIMP-2−/− mice with sponges containing an MMP-14-blocking antibody (BAb; 15 ng/mL) (n = 6 per group; ***P < 0.001 denotes significant difference from all groups; ###P < 0.001 denotes significant difference from TIMP-2−/− mice). Scale bar represents 50 µm and is applicable to all panels.