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. Author manuscript; available in PMC: 2016 Jul 21.
Published in final edited form as: Nature. 2016 Jan 11;529(7586):403–407. doi: 10.1038/nature16480

Figure 1. Nanog induces PGCLCs in EpiLCs.

Figure 1

a, Brightfield/GFP representing D4 male PGCLCs induced by Nanog (+Dox); % GFP+ve cells after FACS; scale bar, 200 μm.

b, FACS plots for GOF-GFP+ve D4 PGCLCs induced by BMP4 or Nanog (+Dox) and +/−Noggin.

c, Analysis of male GOF-GFP cells (qPCR) as indicated. GFP+ve cells were FACS sorted. ΔΔCt +/− s.d. (n=3 biological replicates).

d, Microarray analyses of GOF-GFP ESCs and PGCLCs; unsupervised hierarchical clustering, and principal component (PC)1 scores.

e, IF of Nanog-induced BLIMP1-GFP+ve PGCLCs. Arrowheads highlight single GFP+ve cells; two biological replicates; scale bar, 10 μm; two-sided/unpaired t-test; n.s.=not significant; s=significant; n=number of cells.