FIG 3 .

High-content imaging of DENV infected cells. Huh7 cells were transfected with the indicated siRNAs and infected with DENV2 NGC at an MOI of 1 for 24 h. (A) Representative images used for high-content imaging. Nuclei are stained with Hoechst. Infected cells are indicated by the Alexa Fluor 488 signal from indirect immunofluorescent staining using a primary antibody recognizing the viral envelope protein. (B) Quantification of nuclei per condition by high-content imaging analysis. Data are representative of 4 independent experiments. Error bars represent standard errors of the mean (SEM) for three individual wells. (C) Percentage of cells infected measured by high-content imaging of cells expressing viral envelope protein. Statistical significance measured by Student’s t test: *, P ≤ 0.05; **, P ≤ 0.005; ***, P ≤ 0.0005.