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Measurement of viral titer after host RBP depletion. Huh7 cells were transfected with nonsilencing (NS) control siRNA or siRNA pools containing 4 unique siRNAs per target. Cells were infected with DENV 48 h posttransfection at an MOI of 1. Supernatants were collected 24 h postinfection, and viral titer was determined by focus formation assay. Statistical significance measured by Student’s t test: *, P ≤ 0.05; **, P ≤ 0.005.
