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. 2016 Jan 12;7(1):e02001-15. doi: 10.1128/mBio.02001-15

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Copyright © 2016 Frick-Cheng et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 3 — Immunopurification of HA-CagF from a panel of cag mutant strains. (a) AGS cells were cocultured with the WT strain or the indicated mutants, and IL-8 secretion was quantified by enzyme-linked immunosorbent assay (ELISA). “AGS” indicates cells without added H. pylori. Values represent means ± standard deviations (SD), based on analysis of at least six replicate samples. Levels of IL-8 production induced by mutant strains were compared to levels induced by the WT strain (analysis of variance [ANOVA] followed by Dunn’s multiple-comparison test). *, P < 0.001. (b) HA-CagF was immunoaffinity purified from the strains analyzed in panel A. The affinity-purified samples (immunopurification [IP] elution) were then immunoblotted with the indicated antisera. Strains are designated at the top of the panel, and antisera are designated at the right side of the panel. (c) Summary of results from the experiments presented in panel B, indicating the presence or absence of Cag proteins in the preparations immunopurified from mutant strains. Results are representative of three independent experiments.