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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Pediatr Res. 2015 Sep 2;78(6):650–656. doi: 10.1038/pr.2015.168

Figure 2.

Figure 2

Fluticasone inhibits TNF-α-induced CXCL10, CXCL8, and CCL5 mRNA expression by fetal human airway smooth muscle (ASM) cells. Quiescent fetal ASM cells expressed little CXCL10 (Δ), CXCL8 (X), and CCL5 (□) mRNA under basal conditions (panel a). Stimulation with TNF-α-induced dose-dependent increases in mRNA expression of all three cytokines. Fluticasone (FP, 1 and 100 nmol/l) significantly reduced TNF-α-induced cytokine mRNA expression (panels b–d). Note fetal ASM cells were treated with vehicle or TNF-α (1–20 ng/ml) following pretreatment (1 h) with vehicle or fluticasone. Cytokine mRNA expression was determined 6 h following TNF-α stimulation by RT-PCR as described in the Methods section and expressed as cytokine/β-actin mRNA ratio {plus minus} S.E.M. of three separate experiments, *P < 0.05, **P < 0.01.