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. Author manuscript; available in PMC: 2016 Jan 25.
Published in final edited form as: Immunity. 2015 Apr 21;42(4):731–743. doi: 10.1016/j.immuni.2015.03.012

Figure 2. Id2 dependent microbiota mediates the colonization resistance against C. rodentium infection.

Figure 2

(A–C) 7 weeks old RorccreId2fl/fl (KO) and their littermate Id2fl/fl mice (WT) were treated with either antibiotic (n=5) or control (n=3) in drinking water for one week. One day later, WT and KO mice were orally inoculated with 5 × 106 CFU of C. rodentium. (A) Fecal C. rodentium titers at indicated day post infection. Body weight change (B) and survival rates (C) are shown. Data are representative of two independent experiments.

(D–F) Germ free (GF) wild type B6 mice (D) or GF Ltbr−/− mice (E, F) were colonized with the microbiota from either WT or KO littermate mice by gavage of cecal material. One day later, these mice were orally inoculated with 5 × 106 CFU (D) or 1 × 107 CFU (E, F) of C. rodentium and fecal C. rodentium titers were examined at indicated day post infection (D–E). (F) Survival rates were monitored every day. Data are pooled from three independent experiments.

Each dot represents one individual mouse (A, D, E). Dash line, limit of detection. Error bars represent SEM. *P<0.05, **P<0.01, ***P<0.001; ns, no significant difference (Student’s t-test). See also Figure S2.