Figure 3.

Example of an SDS-PAGE gel used to evaluate the efficiency of antibody cross-linking to Protein A beads. Note this figure has been previously published by Udeshi et al. in ref. 6. TFA eluates from ^~30 ug of pre- and post-cross-linked anti-K-ε-GG antibody were analyzed by SDS-PAGE. More than a 10-fold decrease in staining density for heavy and light chains of the antibody is required.