| MS Method Parameter | Value |
|---|---|
| Polarity | positive |
| Full MS | |
| Microscans | 1 |
| Resolution | 70,000 |
| AGC Target | 3 × 106 ion counts |
| Maximum Ion Time | 10 ms |
| Scan Range | 300-1800 m/z |
| dd-MS2 | |
| Microscans | 1 |
| AGC Target | 5 × 104 ion counts |
| Maximum Ion Time | 120 ms |
| Loop Count | 12 |
| Isolation Window | 2.5 m/z |
| Fixed First Mass | 100 m/z |
| Normalized Collision Energy | 25 |
| dd Settings | |
| Underfill Ratio | 5% |
| Charge Exclusion | unassigned, 1, ≥7 |
| Peptide Match | on |
| Exclude Isotopes | on |
| Dynamic Exclusion | 20 s |
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