Figure 2. Neuro2a cells were incubated with homocysteine (Hcy) for 24 or 48 h. Apoptotic cell populations were assessed by annexin-V and propidium iodide (PI) staining and flow cytometry. A, Representative results of flow cytometry for control and Hcy-treated (5 mM, 48 h) cells. B, Apoptotic cell populations in cells treated with 5 mM Hcy for 0, 24, and 48 h. *P<0.05 compared to 0 h (Student-Newman-Keuls test). C, Apoptotic cell populations in cells treated 48 h with Hcy. *P<0.05 compared to 0 mM (Student-Newman-Keuls test).
