Figure 5. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) acetylation and expression level of p300/CBP protein. Neuro2a cells were incubated in the presence of 5 mM homocysteine (Hcy) or absence (control [con]) of Hcy for 48 h. A, Acetylated proteins in whole cell extracts were immunoprecipitated with acetylated lysine antibody. The GAPDH fractions were then detected by Western blotting. B, Protein levels of p300/CBP in whole cell extracts were detected by Western blotting. *P<0.05 (Student-Newman-Keuls test).