Table 1.
Transcripts examined by qPCR. This table shows the transcripts, tissue and primers used to assess transcript levels in various tissues.
| Transcript | Nerve | DRG | Spinal cord | Primary sequence |
|---|---|---|---|---|
| Ribosomal 18S | + | + | + | 18S-F AATCAGTTATGGTTCCTTTGTC |
| M11188.1 | 18S-R GCTCTAGAATTACCAGTTATCCAA | |||
| α2 A-adrenoreceptor (α2A) | – | + | – | 2A-F GTGTGTTGGTTCCCGTTCTT |
| NM_012739 | 2A-R CGGAAGTCGTGGTTGAAAAT | |||
| α2 C-adrenoreceptor (α2C) | – | + | – | 2C-F GGGTTTCCTCATCGTTTTCA |
| NM_138506 | 2C-R GAAAAGGGCATGACCAGTGT | |||
| Chemokine receptor type 2 (CCR2) | CCR2-F AAGAAGTATCCAAGAGCTTGATGAG | |||
| NM_021866.1 | + | + | – | CCR2-R TCACCATCATCATAGTCATACGG |
| Calcitonin gene related peptide | – | + | – | CGRP-F CCCAGAAGAGATCCTGCAAC |
| AY702025 | CGRP-R GTGGGCACAAAGTTGTCCTT | |||
| GTP cyclohydrolase (GCH1) | – | + | – | GCH1-F AGATTGCAGTGGCCATCAC |
| NM_024356.1 | GCH1-R ACCTCGCATGACCATACACA | |||
| Glial-derived nerve growth factor (GDNF) | – | + | + | GDNF-F GGCTGTCTGCCTGGTGTT |
| NM_019139 | GDNF-R TCAGGATAATTCTTCGGGCATA | |||
| Glutamate (N-methyl-D-Aspartate) receptor-1 (GRIN-1) | – | – | + | GRIN1-F GCTTTTGCAGCCGTAAC |
| NM_017010.1 | GRIN1-R GGGCTCTGCAGCCGTAAC | |||
| Hypoxia-induced factor-1 a (HIF1a) | – | + | + | Hif1a-F AAGCACTAGACAAAGCTCACCTG |
| AC_000074.1 | Hif1a-F CCATATCGCTGTCCACATCA | |||
| Insulin-like growth factor (IGF) | + | + | – | IGF-1-F AGGGCACAGGCTGGCTTTGTAC |
| M15481.1 | IGF-1-R CGATCCAAGTGGCAGCTCCTTC | |||
| Interleukin 1β (IL1β) | + | + | – | IL1b-F CAGGAAGGCAGTGTCACTCA |
| M98820 | IL1b-R AAAGAAGGTGCTTGGGTCCT | |||
| Monocyte chemoattractant protein-1 (MCP1) | + | + | – | MCP1-F AGCATCCACGTGCTGTCTC |
| M57441.1 | MCP1-R GATCATCTTGCCAGTGAATGAG | |||
| Nerve growth factor (NGFβ) | + | + | + | NGFβ-F GTGAGGCTCAGGCAGCAT |
| AC_000070.1 | NGFβ-R TGAGCTTGGGTCCAGCAT | |||
| Neuronal nitric oxide synthase-1 (NOS-1) | + | + | + | nNOS-F GATGAGGCACCCCAACTCT |
| AC_000080.1 | nNOS-R GGAAAGAAACGCAAGGGTTC | |||
| Neurotrophic kinase receptor-1 (nTkr-1) | – | + | – | NTrk1-F CTCGGCTCAGTCACCTGAA |
| NM_021589.1 | NTrk1-R GCACAGTTTTCCAGGAGAGG | |||
| Calcitonin gene-related peptide receptor (rCGRP) | – | + | – | rCGRP-F AGGTCCAGAGGATGAGCAGA |
| NM_053670.3 | rCGRP-R GCTTTGAGCATCACGAACAA | |||
| Tumor necrosis factor-α (TNFα) | + | + | + | TNFα-F ATGTGGAACTGGCAGAGGAG |
| NM_012675 | TNFα-R CAATCACCCCGAAGTTCAGT | |||
| Transient receptor potential cation channel subfamily V member 1 (TrpV1) | – | + | + | Trpv1-F GGTGTGCCTGCACCTAGC |
| Trpv1-R CTCTTGGGGTGGGGACTC | ||||
| NM_031982.1 | ||||
| Transient receptor potential cation channel subfamily V member 4 (TrpV4) | – | + | + | Trpv4-F CTGGTTTACAACAGCAAGATCG |
| Trpv4-R TGTCCCTCAGCAGTTCGTTA | ||||
| NM_023970.1 |