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. Author manuscript; available in PMC: 2016 Jan 25.
Published in final edited form as: J Immunol Methods. 2015 Mar 10;421:27–35. doi: 10.1016/j.jim.2015.02.012
Item Source
Collagenase VIII Sigma-Aldrich
DNase Sigma-Aldrich
Percoll GE Healthcare
10X PBS Gibco
1X PBS Gibco
RPMI-1640 medium with L-glutamine, 500 mL Mediatech
Heat Inactivated Fetal Bovine Serum BenchMark
100X Penicillin/Streptomycin/Glutamine Invitrogen
2-mercaptoethanol Sigma-Aldrich
Glycerol Sigma-Aldrich