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. 2015 Dec 31;53(6):665–673. doi: 10.3347/kjp.2015.53.6.665

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© 2015, Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Interaction of Plasmodium Hsp60 and Plasmodium calpain in the infected RBCs. After isolation of P. falciparum (Pf)-infected RBC (iRBC), the presence of endogenous Pf-Hsp60, GroEL like protein, and Pf-calpain was confirmed by immunoblotting (IB) analysis in uninfected (U) and infected (I) RBC (A). Each GroEL like protein, Pf-Hsp60, and Pf-calpain in iRBC were detected dominantly. Immunoprecipitation (IP) was performed to determine the interaction between Plasmodium Hsp60 and Plasmodium calpain. Lysate (500 μg) of iRBC was immunoprecipitated with anti-Pf-calpin and the presence of Pf-Hsp60 in the eluent was confirmed by IB, using anti-Pf-Hsp60 (B).