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. 2016 Jan 22;36(1):e00286. doi: 10.1042/BSR20150244

Figure 1. Comparison of two fluorescent dyes, safranin and TMRM, during simultaneous measurement of OCR and mtMP in isolated rat cortical mitochondria (200 μg of protein).

Figure 1

Simultaneous assessment of OCR is depicted in A, C, E and mtMP in B, D, F with substrates glutamate (Glu: 10 mM), pyruvate (Pyr: 10 mM), malate (Mal: 2 mM), ADP (2 mM), succinate (Succ: 10 mM), oligomycin (Olig, 1 μM), FCCP (F: 62.5–500.0 nM), rotenone (Rot: 1 μM) and antimycin A (AA: 1 μM). Oxygen concentration levels (nmol/ml) are illustrated in A and C (blue thin lines; left axes) and OCR in [pmol/(s·ml)] in right axes with safranin (thick red line) and TMRM (thick black line) respectively. Fluorescent signal levels of mtMP with safranin and TMRM [(μM), purple thin lines; left axes] and relative signals, mtMP (au) in right axes with safranin (thick red line) and TMRM (thick black line) are in B and D respectively. Levels of OCR and mtMP are also represented in bar charts in E and F with safranin (red) and TMRM (black) respectively.