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. 2016 Jan 22;36(1):e00286. doi: 10.1042/BSR20150244

Figure 7. Estimation of OCR and mtMP with substrates pyruvate and malate for homogenate compared with isolated mitochondria in the presence of TMRM.

Figure 7

OCR in the presence of TMRM are depicted in A with cortical homogenate tissues and in C with cortical isolated mitochondria with sequence additions of pyruvate, malate, ADP, oligomycin, FCCP, rotenone and antimycin A (see Figure 1 for details about concentrations of these chemicals). Oxygen concentration levels (nmol/ml) are illustrated in A and C (blue thin lines; left axes) and OCR in [pmol/(s·ml)] in right axes with homogenate (thick red line) and mitochondria (thick black line) respectively. Fluorescent signal levels of mtMP with homogenate and mitochondria [(μM), purple thin lines; left axes] and relative signals, mtMP (au) in right axes with homogenate (thick red line) and mitochondria (thick black line) are in B and D respectively. Levels of OCR and mtMP are also represented in bar charts in E and F with homogenate (red) and mitochondria (black) respectively.