Skip to main content
. 2016 Jan 22;36(1):e00287. doi: 10.1042/BSR20150215

Figure 6. Effects of hScr3 overexpression on expression of ER stress-responsive XBP1 mRNA.

Figure 6

Control retrovirus-infected (unfilled columns) and hScr3-overexpressed 3T3-L1 cells (grey columns) in triplicate were treated with 2.5 nM Tg for 1 h or 4 h and subjected to RT-qPCR analysis of the expression of total (unspliced and spliced) XBP1 (tXBP1) mRNA (A) and spliced XBP1 (sXBP1) mRNA (B). Relative values compared with control cells on day 0 (set to 1.0) are shown in graph as mean ± S.E.M. (n=3). Ratio of sXBP1 mRNA to tXBP1 mRNA was calculated and is shown in (C). RT-qPCR analysis was also performed to investigate the expression pattern of tXBP1 mRNA (D) and sXBP1mRNA (E) during the course of differentiation of 3T3-L1 cells, and relative values compared with control cells on day 0 (set to 1.0) are shown in graph as mean ± S.E.M. (n=3). Unfilled circles, control cells; grey circles, hScr3-overexpressed cells. Ratio of sXBP1/tXBP1 mRNA is shown in (F). Statistical significance by Student's t test (n=3): *P<0.05; **P<0.01; ***P<0.001, hScr3-overexpressed cells compared with control cells. Statistical significance by Tukey's test (n=3): a0 (P<0.001), Tg treatment for 4 h (panels A and B) or 1 h and 4 h (panel C) compared with that for 0 h in control cells; b0 (P<0.001), Tg treatment for 4 h (panel A) or 1 h and 4 h (panels B and C) compared with that for 0 h in hScr3-overexpressed cells; c0 (P<0.01), day 8 compared with day 0 in control cells; d0 (P<0.05), day 2 (panel D) or days 2 and 8 (panel F) compared with day 0 in hScr3-overexpressed cells; e0 (P<0.001), day 8 compared with day 0 in control cells; f0 (P<0.001), days 2 and 8 compared with day 0 in hScr3-overexpressed cells.