Figure 2. Purified yeast mtHMG proteins differ in their ability to bind dsDNA of various lengths.

(A) Purified mtHMG proteins were visualized by Coomassie staining of gels after their separation by 12% SDS-PAGE. (B) The proteins (at indicated concentrations) were incubated with 3 nM of 15 bp, 25 bp or 50 bp radioactively labelled dsDNA substrates and the samples were separated in polyacrylamide gels as described in Materials and methods. P, free DNA probe; C, DNA–protein complexes.